摘要:采用国标结合振荡法、超声—微波协同萃取法对肉制品中的亚硝酸盐进行提取，并对提取条件进行了单因素及正交试验。结果表明：国标结合振荡法最佳条件：pH为7.5、加热时间15min、料液比1:15、加入水温60℃～70℃、振荡5 min、加入1 g活性炭；超声—微波协同萃取法最佳提取条件：pH为7.5、料液比1:10、提取时间6min、提取功率70W、10%酒精为提取剂、加入1g活性炭。两种方法分别与国标法比较，发现超声—微波协同萃取法的测定结果与国标法测定结果相比较，提取率增加了在3.02%～16.08%之间；国标结合振荡法与国标法测定结果相比较，提取率增加了在0.92%～9.00%之间；超声—微波协同萃取法的测定与国标结合振荡法测定结果相比较，提取率增加了在1.56%～8.00%之间。因此，超声—微波协同萃取法提取亚硝酸盐效果最佳，应用于实际肉制品中亚硝酸盐的提取，熏煮肠类的亚硝酸盐含量在5.1916g～13.7983g之间，压缩火腿类的亚硝酸盐含量在14.2154g～15.9186g之间，相对标准偏差为1.05%～4.30%，加标回收率在96.34%～103.07%之间。
关键词 国标结合振荡法；超声—微波协同萃取法；亚硝酸盐
Abstract:GB combined with the oscillation method, ultrasound-microwave synergistic extraction of nitrite in meat extraction and extraction conditions for a single factor and orthogonal test. The results showed that: GB combined oscillation optimal conditions: pH7.5, heating time 15min, solid-liquid ratio 1:15, water temperature of 60°C ~ 70°C , oscillation for 5min, 1g activated carbon; ultrasound-Microwave Synergistic Extraction The optimum extraction conditions: pH7.5, solid-liquid ratio 1:10, extraction time 6min, extraction power 70W, 10% alcohol as extraction solvent, adding 1g of activated carbon. Two methods, respectively, with the national standard method comparison, found that ultrasound-microwave synergistic extraction method of measuring results compared with the determination of the results of the national standard method, the extraction rate of increase of between 3.02% to 16.08%; GB combined results of the determination of the oscillation method with the country subscript method phase in comparison, the extraction rate of increase between 0.92% to 9.00%; ultrasound-microwave Synergistic Extraction determination GB oscillation method for the determination of the results are compared, the extraction rate of increase of 1.56% ~ 8.00%. Therefore, ultrasound-Microwave Synergistic Extraction to extract the best of nitrite, extraction of nitrite in meat products applied to the actual content of nitrite, smoked cooked sausage in 5.1916g~13.7983g, the content of nitrite in compression ham class in 14.2154g~15.9186g, the relative standard deviation of 1.05% to 4.30%, the recoveries were 96.34%~103.07%.
Keywords GB with oscillation method ultrasonic and microwave synergistic extraction of nitrite
