Hg2+及Hg2+、Cu2+复合离子对蚕豆根尖细胞微核的影响.doc

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摘要:本实验以蚕豆根尖为供试材料,采用实验分析与统计分析的方法,用不同浓度的Hg2+离子(Hg2+离子浓度为5mg/L~100mg/L)及Hg2+、Cu2+复合离子(Hg2+离子浓度为5mg/L~100mg/L,每个浓度分别加入50mg/L的Cu2+离子)污染蚕豆根尖,探讨Hg2+离子及Hg2+、Cu2+复合离子对蚕豆根尖细胞的微核效应。此技术可用于对环境的监测。实验以蒸馏水处理为空白对照,结果表明不同浓度的Hg2+离子及Hg2+、Cu2+复合离子可使蚕豆根尖细胞微核率明显不同,说明Hg2+、Cu2+对豆根尖具有明显的毒性效应——诱发突变(微核) 。并且,随着Hg2+离子浓度的上升,微核率也随之上升(当Hg2+离子溶液浓度在5mg/L~50mg/L之间,随着浓度的上升,微核率随之上升;当Hg2+、Cu2+复合离子溶液浓度在5mg/L~20mg/L之间,随着浓度的上升,微核率也上升),但当达到一定浓度之后(Hg2+离子为50mg/L,微核率为48.51‰;Hg2+、Cu2+复合离子为20mg/L,微核率为19.47‰),微核率开始下降。实验中最低微核率出现在复合离子溶液最高浓度中(100 mg/L)。

关键词:Cu2+; Hg2+; 蚕豆; 根尖细胞; 微核率; 拮抗作用; 细胞遗传学毒理

 

Abstract:This project chooses vicia faba tip as material, by using the methods of experimental analysis and statistical analysis, with different concentrations of Hg2+ (Hg2+ concentration from 5mg / L to100 mg / L)and the combination f Hg2+ and Cu2+ (Hg2+ concentration from 5mg / L to 100 mg / L ,each concentration were added to 50mg/L of Cu2+) pollute vicia faba root tip to explore the micronucleus effect on vicia faba root tip induced by Hg2+ only and the combination of Hg2+ and Cu2+.This technology can be used to monitor the environment.This experiment use distilled water as a blank control test,whose results showed that different concentrations of Hg2+ only and the combination of Hg2+ and Cu2+ can make micronucles rate of vicia faba root tip cells significantly different ,this phenomenon shows that Hg2+ and Cu2+ have cytogenetic toxic effect on the root tip cells of vicia faba root tip cells (micronucles) . And with the rise of Hg2+ concentration, the micronucleus rate rises too (when Hg2+ only concentration between 5mg/L and 50mg/L, with the rise of the concentration, the micronucleus rate rises; when the combination solution of Hg2+ and Cu2+ between 5mg / L and 20 mg / L, with the concentration rise ,the micronucleus rate rises too), but when the concentration reaches a certain level (Hg2+ of 50 mg/L,the micronucleus rate is 48.51‰;the combination of Hg2+ and Cu2+ of 20mg/L,the micronucleus rate is 19.47‰),the rate of micronucleus start to decline. Minimum micronucleus rate in the experiment appear in the highest concentration of the combination solution(100mg/L). 

Keywords: Cu2+; Hg2+; vicia faba; root cells; micronucleus rate; antagonism; toxicology genetics